2′-anilino-6′-[ethyl(3-methylbutyl)amino]-3′-methylspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one CAS:70516-41-5

2'-anilino-6'-[ethyl(3-methylbutyl)amino]-3'-methylspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one serves essential functions as a fluorescent dye in biological staining and fluorescence microscopy, facilitating the visualization and analysis of biological samples with high precision and sensitivity. In biological staining applications, this compound selectively binds to specific cellular structures or biomolecules, allowing for their visualization under fluorescence microscopy. Its fluorescent properties, including high quantum yield and photostability, enable the detection of target molecules with exceptional sensitivity and resolution. By labeling cellular components such as DNA, proteins, and membranes, it enables researchers to study cellular morphology, function, and dynamics, leading to insights into biological processes and disease mechanisms. Moreover, 2'-anilino-6'-[ethyl(3-methylbutyl)amino]-3'-methylspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one finds use in various fluorescence microscopy techniques, including confocal microscopy, super-resolution microscopy, and live-cell imaging. Its compatibility with different imaging modalities allows for versatile applications in biomedical research, ranging from cellular imaging and tissue analysis to drug discovery and development. Furthermore, this compound enables the development of multiplexed imaging assays for simultaneous detection of multiple targets within biological samples. By conjugating it with other fluorophores or biomolecular probes, researchers can create fluorescent probes with distinct emission spectra, facilitating multiplexed imaging of complex biological systems and interactions. Additionally, 2'-anilino-6'-[ethyl(3-methylbutyl)amino]-3'-methylspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one contributes to the advancement of diagnostic techniques in medical and clinical settings. Its use in fluorescence-based assays allows for the detection of biomarkers associated with diseases such as cancer, infectious diseases, and neurological disorders. By providing rapid and sensitive detection of disease biomarkers, it supports early diagnosis, prognosis, and monitoring of therapeutic responses, leading to improved patient outcomes and healthcare management. In summary, 2'-anilino-6'-[ethyl(3-methylbutyl)amino]-3'-methylspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one serves as a versatile fluorescent dye in biological staining and fluorescence microscopy, enabling precise visualization and analysis of biological samples for research, diagnostics, and medical applications. Its unique properties and applications contribute to advancements in biomedical sciences and healthcare.