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2-hydroxy-4-morpholinepropanesulphonic acid CAS:68399-77-9

2-hydroxy-4-morpholinepropanesulphonic acid (CAPS) is a zwitterionic buffering agent commonly used in biochemical and molecular biology experiments. It is an effective pH stabilizer, maintaining a consistent pH in the range of approximately 9.2-10.2. CAPS is particularly known for its applications in protein purification, enzymatic assays, cell culture media, and electrophoresis. It is compatible with enzymes and is often used to maintain the optimal pH for enzyme activity during various laboratory procedures. CAPS is also used in cell culture media to create an optimal environment for cell growth and viability. In electrophoresis, it helps maintain the pH stability necessary for the separation and analysis of nucleic acids or proteins.


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Application and Effect

Buffering: CAPS is widely used as a buffering agent in various biological and biochemical assays. It helps maintain a constant pH, which is critical for the proper functioning of enzymes and other biological molecules.

Protein purification: CAPS is often used as a buffer in protein purification processes. It helps maintain the desired pH for protein stability and activity, enabling efficient purification and preventing protein degradation.

Enzymatic assays: CAPS is frequently utilized in enzymatic assays to provide an optimal pH environment for enzyme activity. It helps ensure accurate and reproducible results by maintaining the pH at the desired level.

Cell culture media: CAPS is commonly added to cell culture media to maintain the pH and provide a stable environment for cell growth and viability. It helps prevent pH fluctuations that can affect cell behavior and experimental outcomes.

Electrophoresis: CAPS is used in electrophoresis to maintain a constant pH during separation and analysis of biomolecules such as nucleic acids or proteins. It aids in achieving accurate and reproducible results in gel-based techniques.

Product Packing:

6892-68-8-3

Additional Information:

Composition C7H15NO5S
Assay 99%
Appearance White powder
CAS No. 68399-77-9
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

 


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