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2-NAPHTHYL-BETA-D-GALACTOPYRANOSIDE CAS:312693-81-5

2-NAPHTHYL-BETA-D-GALACTOPYRANOSIDE is a chemical compound commonly used in biochemical research and analysis. It is a derivative of galactose, a type of sugar. The compound is often used as a substrate for detecting the activity of beta-galactosidase, an enzyme present in many organisms, including bacteria.When beta-galactosidase is present, it cleaves 2-NAPHTHYL-BETA-D-GALACTOPYRANOSIDE into naphthol and galactose. The resulting naphthol molecule can be easily detected by its absorption of ultraviolet light, allowing scientists to measure the activity of beta-galactosidase. This assay is commonly used in molecular biology and genetics research, for applications such as studying gene regulation, protein expression, and cell viability.


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Application and Effect

β-galactosidase reporter assays: The compound is utilized in molecular biology and genetic research as a substrate to measure and quantify beta-galactosidase activity. This is particularly useful in studying gene expression, promoter activity, and protein function.

High-throughput screening: 2-NAPHTHYL-BETA-D-GALACTOPYRANOSIDE can be employed in drug discovery pipelines for the screening of potential enzyme inhibitors or activators. By monitoring the changes in beta-galactosidase activity upon the addition of various compounds, researchers can identify molecules with desired effects.

Monitoring cell viability: The compound can be used as a viability marker to assess cell health and survival. In certain assays, beta-galactosidase activity is an indicator of cell viability, as it is associated with an intact cell membrane and metabolic activity.

Microbiological research: 2-NAPHTHYL-BETA-D-GALACTOPYRANOSIDE is utilized in bacterial identification and selection. Beta-galactosidase produced by some bacteria can hydrolyze the compound, leading to the formation of a blue product, which aids in identifying specific bacterial strains.

Product Packing:

6892-68-8-3

Additional Information:

Composition C16H18O6
Assay 99%
Appearance White crystalline
CAS No. 312693-81-5
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

 


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