2-(tris(hydroxymethyl)methylamino)ethane-1-sulphonic acid CAS:7365-44-8

2-(Tris(hydroxymethyl)methylamino)ethane-1-sulfonic acid, abbreviated as TES, is a chemical compound commonly used as a buffering agent in biological and chemical research. As a sulfonic acid derivative of tris(hydroxymethyl)aminomethane (Tris), TES can maintain a stable pH range of 6.8 to 8.2. It is frequently employed in cell culture media, enzymatic reactions, protein electrophoresis, and DNA/RNA research. TES is highly versatile and crucial for maintaining optimal conditions in various laboratory experiments and techniques.

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Application and Effect

Cell Culture: TES is used in cell culture media to maintain a constant pH, ensuring optimal conditions for the growth and viability of cells. It helps stabilize the pH despite changes caused by metabolic processes or the addition of other reagents.

Enzymatic Reactions: TES is often used as a buffering agent in enzyme assays and biochemical reactions. It helps to maintain the required pH for enzyme activity and stability, ensuring accurate and reproducible results.

Protein Electrophoresis: TES is utilized in protein electrophoresis protocols, such as SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It helps to maintain the correct pH during the separation and analysis of proteins, ensuring accurate migration and detection.

DNA/RNA Research: TES is used in various molecular biology techniques involving DNA and RNA. It helps to stabilize the pH during nucleic acid extraction, purification, and amplification methods like polymerase chain reaction (PCR).

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Composition	C6H15NO6S
Assay	99%
Appearance	White powder
CAS No.	7365-44-8
Packing	Small and bulk
Shelf Life	2 years
Storage	Store in cool and dry area
Certification	ISO.