3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate CAS:75621-03-3

CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) is a commonly used detergent in biochemistry and molecular biology. It is a zwitterionic detergent, meaning it has both a positively and negatively charged group.

CHAPS is known for its ability to solubilize and stabilize membrane proteins, making it useful for various applications such as protein extraction, purification, and characterization. It disrupts lipid-protein interactions, allowing membrane proteins to be extracted in their native state.

Unlike other detergents, CHAPS is relatively mild and does not denature most proteins, making it an excellent choice for maintaining protein structure and function during experiments. It can also help to prevent protein aggregation.

CHAPS is commonly used in techniques like SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), isoelectric focusing, and Western blotting. It is also frequently used in studies involving membrane-bound enzymes, signal transduction, and protein-lipid interactions.

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Application and Effect

Protein Extraction: CHAPS is commonly used to extract membrane proteins from biological samples. It helps solubilize these proteins and maintain their native structure.

Protein Purification: CHAPS is used in various protein purification techniques, such as affinity chromatography. It can be added to purification buffers to solubilize and stabilize membrane proteins during the purification process.

Protein Characterization: CHAPS is often used in studies that involve the characterization of membrane proteins. It helps maintain protein structure and function during experimental procedures like enzyme activity assays, protein-protein interactions, and spectroscopic analyses.

Membrane Protein Studies: Membrane proteins play crucial roles in many cellular processes. CHAPS is commonly used in research related to signal transduction, ion channel function, protein-lipid interactions, and membrane protein crystallization.

Electrophoresis: CHAPS is used in techniques like SDS-PAGE and isoelectric focusing to solubilize membrane proteins and facilitate their separation and analysis.

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Additional Information:

Composition	C32H58N2O7S
Assay	99%
Appearance	White crystalline powder
CAS No.	75621-03-3
Packing	Small and bulk
Shelf Life	2 years
Storage	Store in cool and dry area
Certification	ISO.