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3-Morpholino-2-hydroxypropanesulfonic acid sodium salt CAS:79803-73-9

3-Morpholino-2-hydroxypropanesulfonic acid sodium salt, also known as MES sodium salt, is a chemical compound commonly used as a buffering agent in biological and biochemical research.

MES is a zwitterionic buffer that acts as a pH regulator, keeping the pH stable in various experimental systems. It is highly soluble in water and has a pKa value of approximately 6.15, making it suitable for buffering in the pH range of 5.5 to 7.1.

MES sodium salt is frequently used in molecular biology techniques such as DNA and RNA isolation, enzyme assays, and protein purification. It is also used in cell culture media to maintain a stable pH environment for cell growth and proliferation.

One notable feature of MES is its stability under physiological conditions and resistance to changes in temperature. This makes it suitable for use in experiments where temperature fluctuations are expected.

Researchers often prefer MES sodium salt as a buffer due to its minimal interference with enzymatic reactions and high buffer capacity within its optimal pH range.


Product Detail

Product Tags

Application and Effect

pH Regulation: MES sodium salt acts as a pH regulator, helping to maintain a stable pH environment in experimental systems. It is particularly effective in the pH range of 5.5 to 7.1.

Buffering Capacity: MES has a high buffering capacity within its optimal pH range. It resists changes in pH even when small volumes of acid or base are added, allowing precise control over experimental conditions.

Enzyme Assays: MES is commonly used as a buffer in enzyme assays due to its minimal interference with enzymatic reactions. It helps maintain optimal enzymatic activity by providing a stable pH environment.

Protein Purification: MES buffer is often used in protein purification processes. It helps maintain the stability and activity of proteins during various purification steps, such as ion-exchange chromatography or gel filtration.

DNA and RNA Isolation: MES is used in DNA and RNA isolation procedures, where it helps maintain the stability of nucleic acids and buffers against pH changes that could affect their integrity.

Cell Culture: MES sodium salt is used in cell culture media to maintain a stable pH environment conducive to cell growth and proliferation. It provides a buffered solution that helps maintain optimal conditions for cell culture experiments.

Stability and Compatibility: MES is known for its stability under physiological conditions and resistance to temperature changes. It remains effective in various experimental situations, making it a suitable choice for researchers.

Product Packing:

6892-68-8-3

Additional Information:

Composition C7H16NNaO5S
Assay 99%
Appearance White powder
CAS No. 79803-73-9
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

 


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