3-Morpholino-2-hydroxypropanesulfonic acid sodium salt CAS:79803-73-9

pH Regulation: MES sodium salt acts as a pH regulator, helping to maintain a stable pH environment in experimental systems. It is particularly effective in the pH range of 5.5 to 7.1.

Buffering Capacity: MES has a high buffering capacity within its optimal pH range. It resists changes in pH even when small volumes of acid or base are added, allowing precise control over experimental conditions.

Enzyme Assays: MES is commonly used as a buffer in enzyme assays due to its minimal interference with enzymatic reactions. It helps maintain optimal enzymatic activity by providing a stable pH environment.

Protein Purification: MES buffer is often used in protein purification processes. It helps maintain the stability and activity of proteins during various purification steps, such as ion-exchange chromatography or gel filtration.

DNA and RNA Isolation: MES is used in DNA and RNA isolation procedures, where it helps maintain the stability of nucleic acids and buffers against pH changes that could affect their integrity.

Cell Culture: MES sodium salt is used in cell culture media to maintain a stable pH environment conducive to cell growth and proliferation. It provides a buffered solution that helps maintain optimal conditions for cell culture experiments.

Stability and Compatibility: MES is known for its stability under physiological conditions and resistance to temperature changes. It remains effective in various experimental situations, making it a suitable choice for researchers.