3-morpholinopropanesulfonic acid hemisodium salt CAS:117961-20-3

pH buffering: MOPS-Na is effective at maintaining a stable pH in the physiological range (pH 6.5-7.9). Its zwitterionic nature allows it to resist changes in pH when acids or bases are added, making it useful for maintaining optimal conditions in cell culture media and various experimental systems.

Protein and enzyme studies: MOPS-Na is often used in protein purification, characterization, and stabilization. Its buffering capacity and compatibility with many enzymes and proteins make it suitable for maintaining the desired pH during these processes. MOPS-Na can also be employed in enzyme assays, where precise pH control is essential for accurate measurement of enzymatic activity.

DNA and RNA electrophoresis: MOPS-Na is commonly used as a buffer in nucleic acid gel electrophoresis. It provides the desired pH range and ionic strength, allowing for efficient separation of DNA and RNA fragments. The low UV absorption of MOPS-Na is advantageous in this application, as it does not interfere with spectrophotometric measurements of nucleic acids.

Cell culture media: MOPS-Na is utilized in cell culture media to maintain the pH and osmotic balance necessary for cell growth and viability. Its compatibility with various cell types and non-toxic nature make it an ideal buffering agent for maintaining physiological conditions in cell culture experiments.

Pharmacological and biological research: MOPS-Na is employed in various pharmacological and biological studies, such as measurement of enzyme kinetics, drug screening assays, and studies involving the effects of pH on cellular processes. Its buffering capacity helps to minimize pH-dependent artifacts and ensure reliable and reproducible results.