3,3 ,5,5 -Tetramethylbenzidine dihydrochloride hydrate CAS:207738-08-7

3,3',5,5'-Tetramethylbenzidine dihydrochloride (TMB) is a widely used substrate in enzyme-linked immunosorbent assays (ELISAs) and other biochemical assays. When TMB is oxidized by the enzyme horseradish peroxidase (HRP) in the presence of hydrogen peroxide, it produces a blue color. This color change is used to detect the presence of HRP-labeled antibodies or other proteins, often indicating the presence of a specific antigen in a sample.The blue color generated by the reaction is typically quantified using a spectrophotometer, which allows for the measurement of the intensity of the color change and provides a means to correlate the color intensity with the amount of target molecule present in the sample.TMB has numerous applications in diagnostic assays, research, and pharmaceutical development, particularly in the detection of antibodies, hormones, cytokines, and other biomolecules. Its sensitivity, stability, and ease of use make it a valuable tool in various laboratory settings for the detection and quantification of specific molecules.