3,3′,5,5′-Tetramethylbenzidine CAS:207738-08-7

3,3′,5,5′-Tetramethylbenzidine, also known as TMB, is a chemical compound commonly used as a chromogenic substrate in enzyme-linked immunosorbent assays (ELISA) and other biochemical assays. It is often used to detect and quantify the presence of enzymes such as horseradish peroxidase (HRP) in various biological samples. TMB undergoes a color change from colorless to blue in the presence of these enzymes. Subsequently, the reaction can be stopped by adding an acid which converts the blue color to a final yellow color. The intensity of the yellow color is proportional to the amount of enzyme present, allowing for quantification.

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Application and Effect

ELISA: TMB is often used in ELISA-based assays to detect and quantify the presence of proteins, antibodies, or antigens. The intensity of the color is directly proportional to the concentration of the target molecule.

Enzyme activity assays: TMB can be used to measure the activity of enzymes like HRP. By measuring the rate of color change, researchers can determine the activity or concentration of the enzyme in a sample.

Biological staining: TMB can be used in immunohistochemistry and immunocytochemistry to visualize specific tissue or cell components. When TMB reacts with the enzyme HRP, it produces a brown or blue precipitate at the site of the target molecule, allowing for its visualization under a microscope.

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Additional Information:

Composition	C16H23ClN2O
Assay	99%
Appearance	White powder
CAS No.	207738-08-7
Packing	Small and bulk
Shelf Life	2 years
Storage	Store in cool and dry area
Certification	ISO.