3xflag CAS:63415-74-7

3xFlag peptide serves as a versatile tool in molecular biology, particularly for the identification, purification, and characterization of recombinant proteins. The 3xFlag tag, composed of three repeats of the Flag peptide sequence (DYKDDDDK), provides enhanced detection capabilities through increased binding affinity for specific antibodies. This makes it particularly useful in various experimental applications, including Western blotting, immunoprecipitation, and mass spectrometry. Researchers utilize the 3xFlag tag to monitor protein expression levels and analyze subcellular localization. By tagging proteins of interest, scientists can easily track their presence in different cellular compartments and study their functional roles in signaling pathways and protein interactions. The 3xFlag tag facilitates the use of affinity chromatography, allowing for efficient purification of tagged proteins from complex mixtures, thereby improving yields for downstream applications. Moreover, 3xFlag is instrumental in studying protein-protein interactions, enabling researchers to conduct co-immunoprecipitation assays and other interaction studies. The increased sensitivity of detection afforded by the 3xFlag tag enhances the ability to identify low-abundance proteins and transient interactions, which are often missed with conventional tagging methods. Overall, the 3xFlag peptide tag is an invaluable asset in the fields of cell biology, biochemistry, and molecular genetics, driving advancements in our understanding of cellular processes and facilitating the development of therapeutic strategies.