4-Amino-3-hydrazino-1,2,4-triazol-5-thiol CAS:28836-03-5

The effect of 3,3'-Diaminobenzidine (DAB) is to produce a brown color when it reacts with peroxidase enzymes. This color change allows for the visualization of target antigens or biomolecules of interest in immunohistochemistry (IHC) and immunocytochemistry (ICC) staining assays.DAB is often used in biomedical research and diagnostic applications to detect and localize specific antigens or proteins within biological samples. By adding DAB as a substrate and subsequent reaction with peroxidase enzymes, a brown precipitate is formed at the site of the target antigens.The application of DAB staining is particularly useful in studying various diseases and conditions. It enables researchers and pathologists to visualize and analyze the distribution, abundance, and localization of specific biomolecules in tissues or cells. This information aids in understanding disease mechanisms, studying cellular processes, and identifying potential diagnostic or therapeutic targets.Moreover, DAB staining can be used in different molecular techniques to investigate protein-protein interactions, identify protein expression patterns, determine the presence of specific markers, and evaluate the effectiveness of therapeutic interventions.Due to its reliable and robust staining, as well as its compatibility with different detection systems and microscopy techniques, DAB has become a widely used chromogenic substrate in immunohistochemistry and immunocytochemistry assays.