4-Aminoantipyrine CAS:83-07-8

Colorimetric Assays: 4-Aminoantipyrine is commonly used as a chromogenic reagent in colorimetric assays in analytical chemistry. It reacts with specific substances, such as phenols and peroxides, to form colored complexes. These colored complexes can be measured spectrophotometrically, allowing for the quantitative determination of various analytes.

Enzyme Determination: 4-Aminoantipyrine is often utilized in enzyme assays for the detection and quantification of enzymes like peroxidase, glucose oxidase, and amylase. It acts as a substrate for these enzymes and undergoes a chemical reaction that produces a colored compound. The intensity of the color is directly proportional to the activity or concentration of the enzyme being measured.

Pharmaceutical Industry: In the past, 4-Aminoantipyrine was used as an analgesic and antipyretic (fever-reducing) drug. However, its usage as a medication has significantly decreased due to safety concerns and the availability of alternative drugs with fewer adverse effects.

Research and Development: 4-Aminoantipyrine is also utilized in various research and development applications, particularly in the pharmaceutical and biomedical fields. It can be employed as a reagent in the synthesis of new drug compounds or as a tool in studying enzyme kinetics and mechanisms.