4-Fluoro-7-nitrobenzofurazan CAS:29270-56-2

Fluorescent Probe: FNB is commonly used as a fluorescent probe. It reacts with primary amines to form highly fluorescent products. This property allows FNB to be used for the labeling and detection of proteins, peptides, and other amino-containing compounds.

Amino Acid Analysis: FNB is crucial in amino acid analysis techniques, specifically in the derivatization of amino acids. It reacts selectively with primary amines in amino acids, forming derivatives that can be separated and quantified by chromatographic techniques.

Enzyme Kinetics: FNB is utilized for studying enzyme kinetics. It can be used to measure the reaction rates of enzymes that produce or consume primary amines. The change in fluorescence intensity of the FNB derivative during the enzymatic reaction can provide valuable information about enzyme activity.

Nucleic Acid Sequencing: FNB is employed in nucleic acid sequencing methods to detect and identify specific nucleotides. It can react specifically with primary amines in nucleotides, allowing for the determination of nucleotide sequences during the sequencing process.