4-Morpholineethanesulfonic acid CAS:4432-31-9

pH Buffering: MES has a pKa value around 6.1, making it an effective buffer in the pH range of 5.5 to 6.7. It helps maintain a stable pH by resisting changes in acidity or alkalinity. This is particularly useful in experiments and assays that require a specific pH environment.

Enzyme Studies: MES is commonly used in enzyme research and assays due to its compatibility with various enzymes. It helps maintain optimal pH conditions for enzyme activity, ensuring accurate and reliable results.

Protein Purification: MES is used in protein purification processes, such as chromatography, to maintain the stability and activity of the target protein. It aids in maintaining the protein's native conformation and functionality during purification steps.

Electrophoresis: MES is frequently used in gel electrophoresis procedures, especially for separating and analyzing smaller proteins and peptides. Its buffering capacity ensures a stable pH, which is essential for accurate visualization and characterization of protein bands.

Cell Culture: MES is commonly used in cell culture studies and media formulations as a buffering agent. It helps maintain the pH within the optimal range for cell growth, viability, and biochemical processes without interfering with cellular functions.

Chemical Reactions: MES can also be used as a reagent in chemical reactions since it can act as a weak base or acid. Its buffering capacity helps maintain a constant pH during the reaction, enabling better control and reproducibility.