4-NITROPHENYLΑ-D-MALTOHEXAOSIDE CAS:74173-30-1

Determining enzyme activity: 4-nitrophenyl α-D-maltohexaoside is used to evaluate the activity of enzymes such as α-amylases, glucosidases, and glycosyltransferases. These enzymes cleave the α-glycosidic bond within the compound, producing a nitrophenol product that can be easily measured.

Substrate specificity: By using 4-nitrophenyl α-D-maltohexaoside, scientists can investigate the substrate specificity of enzymes. It allows them to determine which enzymes can specifically recognize and cleave α-glycosidic bonds within maltohexaoside.

Kinetic studies: The compound is also used to determine the kinetic parameters (e.g., Km, Vmax) of enzymes involved in carbohydrate metabolism. By measuring the rate of nitrophenol release, researchers can obtain information about the enzyme's substrate affinity and catalytic efficiency.

Mechanistic studies: 4-nitrophenyl α-D-maltohexaoside is employed in mechanistic studies to investigate the reaction mechanisms of enzymes involved in carbohydrate metabolism. It helps to elucidate the stepwise events that occur during enzyme-catalyzed reactions.

High-throughput screening: In drug discovery and biotechnological applications, 4-nitrophenyl α-D-maltohexaoside can be used in high-throughput screening assays to identify potential enzyme inhibitors or activators. This compound allows for the rapid testing of large libraries of compounds for their impact on enzyme activity.