4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS:3459-18-5

Enzyme Substrate: pNAG is widely used as a specific substrate for various enzymes involved in the hydrolysis of β-D-glucoside bonds. When these enzymes cleave the pNAG molecule, it releases p-nitrophenol. This allows researchers to measure and quantify the enzymatic activity.

Enzyme Activity Assays: The hydrolysis of pNAG by specific enzymes can be detected and measured spectrophotometrically. This makes pNAG suitable for enzyme activity assays, where the amount of p-nitrophenol generated is directly proportional to the enzymatic activity.

High-Throughput Screening: pNAG is commonly used in high-throughput screening assays to identify and characterize enzyme inhibitors or activators. By evaluating the effect of different compounds on the enzymatic activity, researchers can identify potential drug candidates or modulators of enzyme function.

Gene Expression Studies: pNAG is also used in molecular biology research to study gene expression and regulation. By measuring the enzymatic activity of specific enzymes using pNAG as a substrate, researchers can investigate the effect of gene expression on enzyme function and activity.