4-Nitrophenyl-alpha-D-glucopyranoside CAS:3767-28-0

Substrate for enzymatic assays: 4-Nitrophenyl-alpha-D-glucopyranoside is commonly used as a substrate in enzymatic assays to measure the activity of glycosidases and other enzymes involved in carbohydrate metabolism. These enzymes can cleave the bond between the glucose and 4-nitrophenyl group, releasing a yellow product called 4-nitrophenol. The amount of 4-nitrophenol generated is directly proportional to the enzyme activity, allowing for the quantitative measurement of enzyme activity.

Detection of enzyme activity: By using 4-Nitrophenyl-alpha-D-glucopyranoside as a substrate, the activity of specific enzymes can be measured. For example, glycosidase enzymes such as beta-glucosidase, alpha-glucosidase, or beta-galactosidase can cleave the compound, releasing 4-nitrophenol, which can be detected photometrically. This method is widely used in various medical, pharmaceutical, and biotechnological applications.

High-throughput screening: Due to its compatibility with automated detection systems, 4-Nitrophenyl-alpha-D-glucopyranoside is commonly used in high-throughput screening assays. This compound allows for the rapid and efficient characterization of enzyme activity, making it useful in drug discovery, enzyme engineering, and enzyme inhibition studies.

Diagnostic applications: 4-Nitrophenyl-alpha-D-glucopyranoside can be utilized in diagnostic applications to detect the presence or activity of specific enzymes related to various diseases. For example, the activity of certain enzymes can be used as a diagnostic marker for specific diseases, and 4-Nitrophenyl-alpha-D-glucopyranoside can be used as a substrate to measure the activity of these enzymes in clinical samples.