4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Enzyme substrates: 4NPM can be used as a substrate for various enzymes, including glycosidases and related enzymes. These enzymes cleave the glycosidic bond between the mannose and 4NPM, resulting in the release of the nitrophenyl moiety. The extent of substrate hydrolysis can be measured spectrophotometrically by monitoring the absorbance of the released nitrophenyl group at a specific wavelength. This allows researchers to assess the enzyme activity and kinetics.

Assays for carbohydrate metabolism: By using 4NPM as a substrate, researchers can study the activity of enzymes involved in carbohydrate metabolism, such as alpha-mannosidases. These enzymes hydrolyze glycosidic bonds in mannose-containing compounds, and their activity can be measured by monitoring the release of the nitrophenyl moiety from 4NPM.

Glycosylation studies: 4NPM can also be used in assays to investigate enzymes involved in glycosylation processes. Glycosylation is the process of attaching sugar molecules to proteins or other molecules, and many enzymes are involved in this process. By using 4NPM as an acceptor substrate, researchers can measure the transfer of a sugar moiety to 4NPM by specific enzymes involved in glycosylation reactions.

Screening for enzyme inhibitors or activators: 4NPM can be used in high-throughput screening assays to identify compounds that inhibit or activate specific enzymes. By assessing the effect of test compounds on the hydrolysis or modification of 4NPM by the target enzymes, researchers can identify potential therapeutic agents or useful chemical probes for studying enzyme function.