4-Nitrophenyl-alpha-L-fucopyranoside CAS:10231-84-2

Enzyme activity assay: 4-Nitrophenyl-alpha-L-fucopyranoside is widely used as a substrate in enzymatic assays to determine the activity of fucosidase enzymes. Fucosidases are involved in breaking down fucose-containing molecules, and by measuring the release of 4-nitrophenol, the activity of these enzymes can be quantitatively determined.

Substrate specificity studies: By using 4-Nitrophenyl-alpha-L-fucopyranoside as a substrate, researchers can investigate the specificity of fucosidase enzymes towards different substrates. This can help in understanding the role of fucosidases in various biological processes and pathways.

Inhibitor screening: 4-Nitrophenyl-alpha-L-fucopyranoside can be used to screen and evaluate potential inhibitors of fucosidase enzymes. This is important in drug development and therapeutic interventions, as inhibiting the activity of specific enzymes can have pharmacological effects.

Kinetics studies: By observing the reaction rate of fucosidase enzymes with 4-Nitrophenyl-alpha-L-fucopyranoside at different substrate concentrations and enzyme concentrations, the kinetics of the enzymatic reaction can be studied. This can provide insights into the mechanism of the enzyme and its interactions with the substrate.