4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7

Enzyme activity assay: The hydrolysis of 4-Nitrophenyl-beta-D-glucopyranoside by enzymes like β-glucosidase can be used to measure enzyme activity. The release of 4-nitrophenol can be quantified using spectrophotometry, allowing for the determination of enzyme kinetics and inhibition studies.

Gene expression and reporter assays: 4-Nitrophenyl-beta-D-glucopyranoside can be coupled with specific molecular tags that are incorporated into fusion proteins. The enzyme activity of the fusion protein can then be easily quantified by measuring the release of 4-nitrophenol, providing insights into gene expression and protein-protein interactions.

Drug discovery and high-throughput screening: The hydrolysis of 4-Nitrophenyl-beta-D-glucopyranoside can be used as a surrogate activity assay for drug discovery. It allows for the rapid screening of large compound libraries to identify potential enzyme inhibitors or activators.

Diagnostic testing: Some diseases are associated with alterations in certain enzyme activities. By utilizing the hydrolysis of 4-Nitrophenyl-beta-D-glucopyranoside by specific enzymes as a diagnostic test, these diseases can be detected or monitored. This includes conditions related to lysosomal storage disorders and hepatobiliary diseases.