4-Nitrophenyl-beta-D-xylopyranoside CAS:2001-96-9

The effect of 4-nitrophenyl-beta-D-xylopyranoside is to act as a substrate for the enzyme beta-xylosidase. This enzyme catalyzes the hydrolysis of the substrate, resulting in the release of 4-nitrophenol. The release of 4-nitrophenol leads to a color change from colorless to yellow.

The application of 4-nitrophenyl-beta-D-xylopyranoside is primarily in enzymatic assays to measure beta-xylosidase activity. This substrate is commonly used in research laboratories and pharmaceutical industries to study the kinetics and inhibition of beta-xylosidase enzymes. By measuring the amount of 4-nitrophenol produced, researchers can quantify the enzymatic activity and characterize the enzyme's properties.