4-Nitrophenyl phosphate disodium salt hexahydrate CAS:4264-83-9

4-Nitrophenyl phosphate disodium salt hexahydrate is a chemical compound that is commonly used as a substrate for detecting the activity of phosphatase enzymes. It appears as a white to off-white powder and is highly soluble in water. When acted upon by phosphatase enzymes, it undergoes a reaction that results in the production of a yellow color, which can be measured spectrophotometrically. This compound finds applications in various biochemical assays and diagnostic kits for detecting and quantifying phosphatase activity in samples.

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Application and Effect

Enzyme activity assays: It is commonly used to assess the activity of alkaline phosphatase and other phosphatase enzymes in biological samples. The rate of yellow color formation can be measured to determine enzyme activity levels.

Diagnostic kits: It is used as a substrate in diagnostic kits, particularly for the detection of alkaline phosphatase activity in clinical samples. These kits can be used to diagnose diseases related to abnormal phosphatase activity, such as liver dysfunction or bone disorders.

Drug discovery and development: It can be used in high-throughput screening assays to evaluate the effect of various compounds on phosphatase activity. This can aid in the discovery and development of potential therapeutic agents targeting phosphatase enzymes.

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Additional Information:

Composition	C6H7NNaO6P
Assay	99%
Appearance	White powder
CAS No.	4264-83-9
Packing	Small and bulk
Shelf Life	2 years
Storage	Store in cool and dry area
Certification	ISO.