4-Nitrophenyl phosphate di(tris) salt CAS:68189-42-4

4-Nitrophenyl phosphate di(tris) salt is commonly used as a substrate for alkaline phosphatase in various biochemical and enzyme assays. When hydrolyzed by alkaline phosphatase, it produces a yellow-colored product that can be easily measured spectrophotometrically. This reaction is widely utilized in applications such as enzyme-linked immunosorbent assays (ELISAs) and phosphatase activity detection.

The yellow color intensity produced is directly proportional to the enzyme activity, allowing for accurate quantification of alkaline phosphatase levels in biological samples. This makes it useful in medical and research settings for studying alkaline phosphatase activity in cells, tissues, and biological fluids.

Additionally, 4-Nitrophenyl phosphate di(tris) salt is stable and highly water-soluble, enhancing its suitability for various experimental conditions. Its high sensitivity and ease of measurement make it a popular choice as a substrate in enzyme assays, aiding in the evaluation of enzyme kinetics, enzyme activity, and drug discovery.