5-Bromo-4-chloro-3-indolyl phosphate disodium salt CAS:102185-33-1

Immunohistochemistry (IHC): BCIP is often used in IHC assays to visualize the presence or localization of specific antigens in tissue samples. The blue precipitate produced by BCIP allows for the identification of antigenic sites and can be used for qualitative or semi-quantitative analysis.

In situ hybridization (ISH): BCIP is commonly employed in ISH techniques to detect and localize specific nucleic acid sequences, such as mRNA or DNA. The blue precipitate formed by BCIP indicates the presence of the target sequences and aids in the visualization of their distribution within cells or tissues.

Enzyme-linked immunosorbent assays (ELISAs): BCIP is sometimes used in ELISAs to detect the presence or concentration of specific biomolecules, such as proteins or antibodies. By conjugating BCIP with an appropriate enzyme label and using alkaline phosphatase to catalyze the reaction, the blue color produced can indicate the presence and abundance of the biomolecule of interest.