5-Bromo-6-Chloro-3-Indolyl Acetate CAS:102185-48-8

Enzyme activity assays: Researchers use this compound to assess the activity of various enzymes in biological samples. By measuring the formation of the colored or fluorescent product, they can quantify enzyme activity and compare it across different samples or experimental conditions.

Screening for enzyme inhibitors: 5-Bromo-6-chloro-3-indoxyl-3-acetate can be used in high-throughput screening studies to identify potential inhibitors of specific enzymes. By monitoring the reduction or inhibition of the colored or fluorescent signal, researchers can select compounds that modulate the activity of the target enzyme.

Gene expression analysis: Given its ability to detect enzyme activity, 5-Bromo-6-chloro-3-indoxyl-3-acetate can also be utilized to study the expression of genes coding for specific enzymes. By measuring the enzymatic activity in tissues or cells, researchers can infer the level of gene expression and investigate its regulation.

Clinical diagnostics: This compound can be employed in clinical assays to diagnose certain diseases or conditions. For example, it is commonly used to detect the presence of enzyme activity associated with certain bacterial or fungal infections.