5-Sulfosalicylic acid dihydrate CAS:5965-83-3

Protein precipitation: 5-Sulfosalicylic acid dihydrate is often used to precipitate proteins from biological samples. It can help in the isolation and purification of proteins for further analysis. The acid's ability to form insoluble complexes with proteins makes it useful in this regard.

Quantification of proteins: 5-Sulfosalicylic acid dihydrate can be utilized in assays to measure the concentration of proteins in biological samples like urine, serum, or cell lysates. It forms a stable complex with proteins, allowing their quantification through colorimetric or spectrophotometric methods.

Nucleic acid isolation: The acid can also be employed in nucleic acid extraction procedures. It aids in precipitating proteins and other impurities, leaving behind purified nucleic acids that can be further analyzed or used in molecular biology experiments.

Enzyme assays: 5-Sulfosalicylic acid dihydrate is commonly used to measure enzyme activity. It can help in the determination of enzyme kinetics, inhibition studies, and other enzymatic assays by precipitating proteins and interfering substances, allowing specific measurement of enzyme activity.

Drug analysis: The acid can be employed in drug assays, particularly for drugs that form complexes with proteins. It helps isolate and quantify drugs by precipitating the protein-drug complexes, allowing their separation from other interfering substances.

Diagnostic applications: 5-Sulfosalicylic acid dihydrate is used in various diagnostic techniques, such as protein urine tests or screening for certain diseases. It allows the detection or quantification of specific proteins or biomarkers related to certain medical conditions.