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8-Anilino-1-naphthalenesulfonic acid ammonium salt CAS:28836-03-5

8-Anilino-1-naphthalenesulfonic acid ammonium salt is a chemical compound that is commonly used as a fluorescent dye. It has a structure consisting of an aniline group attached to a naphthalenesulfonic acid molecule with an ammonium salt. This compound is soluble in water and exhibits a strong absorption and emission in the visible light range.

Due to its fluorescent properties, 8-Anilino-1-naphthalenesulfonic acid ammonium salt is often used as a probe or indicator in various biochemical and biophysical studies. It can be used to detect changes in pH, conformational changes in proteins, protein-ligand interactions, and the presence of certain ions.

When excited with an appropriate wavelength of light, this compound emits a bright blue fluorescence, allowing for easy detection and measurement. Its fluorescence properties make it a valuable tool in research areas such as molecular biology, biochemistry, and cell biology.


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Application and Effect

pH indicator: The compound undergoes a shift in its fluorescence emission peak depending on the pH of the solution it is in. This property makes it useful as a pH indicator in various applications, such as monitoring pH changes in biological samples or studying enzymatic reactions.

Protein conformation analysis: 8-Anilino-1-naphthalenesulfonic acid ammonium salt can bind to proteins and undergo a change in fluorescence intensity or spectral properties depending on the conformation of the protein. This makes it a useful tool for studying protein folding, denaturation, and conformational changes.

Protein-ligand interactions: The compound can also be used to study protein-ligand interactions. By binding to a protein and undergoing a change in fluorescence upon ligand binding, it allows researchers to investigate the binding affinity, kinetics, and mechanism of action of different ligands.

Ion detection: 8-Anilino-1-naphthalenesulfonic acid ammonium salt has been used to detect and quantify various metal ions, such as calcium, magnesium, and zinc. The fluorescence properties of the compound can be modulated by the presence of these ions, providing a sensitive and selective method for their detection.

Environmental monitoring: The compound has been employed for environmental monitoring purposes, such as detecting pollutants and monitoring changes in water quality. Its fluorescence properties enable rapid and sensitive detection of contaminants in water samples.

Analytical chemistry: 8-Anilino-1-naphthalenesulfonic acid ammonium salt is also used in various analytical techniques, such as fluorescence spectroscopy and high-performance liquid chromatography (HPLC), for the quantification and characterization of compounds in complex mixtures.

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试剂包装2

Additional Information:

Composition C16H16N2O3S
Assay 99%
Appearance Green powder
CAS No. 28836-03-5
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

 


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