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8-Anilino-1-naphthalenesulfonic acid CAS:82-76-8

8-Anilino-1-naphthalenesulfonic acid, also known as ANS, is a fluorescent dye commonly used in biochemical and biophysical research. It belongs to the class of organic compounds known as aniline-based sulfonic acids. ANS is known for its ability to bind to hydrophobic regions or exposed hydrophobic sites in proteins and other biomolecules.

ANS fluoresces when it is bound to hydrophobic regions, making it a useful probe for studying protein conformational changes, protein-protein interactions, and protein folding/unfolding processes. The binding of ANS to hydrophobic regions leads to a blue shift in the emission spectrum, which can be monitored using fluorescence spectroscopy.

ANS is often used in a variety of research techniques, including fluorescence polarization, fluorescence resonance energy transfer (FRET), and thermal stability assays. It has been utilized to study the structure and stability of proteins, protein-ligand interactions, and protein aggregation processes.


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Application and Effect

Fluorescent probe: ANS is widely used as a fluorescent probe to study protein conformational changes, protein folding/unfolding processes, and protein-ligand interactions. It binds to hydrophobic regions or exposed hydrophobic sites in proteins, resulting in a fluorescence emission that can be monitored using fluorescence spectroscopy.

Hydrophobicity detection: ANS is sensitive to changes in the hydrophobicity of the microenvironment. It can be used to detect and quantify surfactants, detergents, and other hydrophobic substances.

Protein stability assessment: ANS is commonly employed in thermal stability assays to assess the stability of proteins. By monitoring the fluorescence intensity of ANS-bound proteins under different temperatures, it is possible to determine the thermal denaturation characteristics and melting temperature (Tm) of the protein.

Protein aggregation studies: ANS can be utilized to study protein aggregation processes, such as the formation of amyloid fibrils. The dye is sensitive to the exposed hydrophobic regions in protein aggregates, making it a valuable tool for monitoring the progress and kinetics of aggregation.

Characterization of protein-protein interactions: ANS has been used to investigate protein-protein interactions. When proteins interact, there may be a change in their hydrophobicity, which can be monitored using ANS fluorescence.

Drug discovery and development: ANS can be utilized in the screening of potential drug candidates. It can help evaluate the binding affinity and interaction of small molecules with target proteins, allowing researchers to identify compounds that can modulate protein function.

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Additional Information:

Composition C16H13NO3S
Assay 99%
Appearance Dark green powder
CAS No. 82-76-8
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

 


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