ABTS (2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt) CAS:30931-67-0

Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), often referred to as ABTS, is a commonly used chromogenic substrate in biochemical assays, particularly in the field of enzymology. It is a synthetic compound that is used to measure the activity of various enzymes, including peroxidases and oxidases.

ABTS is colorless in its oxidized form but turns blue-green when oxidized by an enzyme in the presence of hydrogen peroxide or molecular oxygen. This color change is due to the formation of a radical cation, which absorbs light in the visible spectrum.

The reaction between ABTS and the enzyme produces a colored product that can be measured spectrophotometrically. The intensity of the color is directly proportional to the enzymatic activity, allowing researchers to quantitatively evaluate enzyme kinetics, enzyme inhibition, or enzyme-substrate interactions.

ABTS has a broad range of applications in various fields, including clinical diagnostics, pharmaceutical research, and food science. It is highly sensitive and offers a wide dynamic range, making it a popular choice for many biochemical assays.

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Application and Effect

Enzymatic Assays: ABTS is widely used to measure the activity of enzymes such as peroxidases and oxidases. It acts as a substrate for these enzymes, and their activity can be quantified by measuring the intensity of the colored product formed.

Antioxidant Capacity Assays: ABTS is often employed in antioxidant capacity assays to determine the ability of substances to scavenge or inhibit free radicals. The color formation in the presence of an antioxidant is indicative of its radical scavenging capacity.

Protein Assays: ABTS can be used to assess total protein content in biological samples. The reaction of ABTS with protein-bound copper results in the formation of a colored product that can be quantified. This method is commonly known as the bicinchoninic acid (BCA) assay.

Drug Discovery: ABTS is used in high-throughput screening assays to evaluate the antioxidant activities of potential drug compounds. This allows researchers to identify compounds with potential therapeutic effects.

Food and Beverage Industry: ABTS is used in the food and beverage industry to assess the antioxidant capacity of various food products, such as fruits, vegetables, and beverages. It helps evaluate the potential health benefits and stability of these products.

Environmental Monitoring: ABTS can be employed to assess the total antioxidant capacity of environmental samples, aiding in the evaluation of pollutant levels and their impact on the environment.

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Additional Information:

Composition	C18H24N6O6S4
Assay	99%
Appearance	Green powder
CAS No.	30931-67-0
Packing	Small and bulk
Shelf Life	2 years
Storage	Store in cool and dry area
Certification	ISO.