AMPPD CAS:122341-56-4 Manufacturer Price

AMPPD, short for 3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane, is a chemiluminescent substrate commonly used in biological and immunoassay applications. It is widely used in molecular biology research, diagnostics, and life sciences.

AMPPD is specifically designed to be used in combination with alkaline phosphatase enzyme conjugates. When exposed to alkaline phosphatase, AMPPD undergoes a rapid and efficient enzymatic cleavage reaction, leading to the emission of light.

The emitted light from AMPPD is detected using sensitive imaging systems or luminometers, allowing for the quantification and visualization of target molecules or analytes. This chemiluminescence-based detection method offers high sensitivity, low background noise, and a broad dynamic range.

Due to its stability and long-lasting signal, AMPPD is well-suited for applications such as Western blotting, ELISA (enzyme-linked immunosorbent assay), DNA hybridization, and in situ hybridization. It enables researchers to detect and analyze proteins, nucleic acids, antibodies, and other biomolecules.

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Application and Effect

AMPPD is typically utilized in combination with the enzyme alkaline phosphatase. When exposed to alkaline phosphatase, AMPPD undergoes a cleavage reaction that generates a light signal. This light emission can be detected and measured using specialized instruments, enabling the visualization and quantification of target biomolecules.

The application of AMPPD is widespread in various techniques, including Western blotting, ELISA (enzyme-linked immunosorbent assay), DNA and RNA hybridization, and in situ hybridization. These techniques are employed to study proteins, nucleic acids, antibodies, and other biological molecules.

AMPPD provides several advantages, including high sensitivity, low background noise, and a wide dynamic range. Its stable and long-lasting signal makes it suitable for both qualitative and quantitative analyses.

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Additional Information:

Composition	C16H28N6O10P2
Assay	99%
Appearance	White powder
CAS No.	122341-56-4
Packing	Small and bulk
Shelf Life	2 years
Storage	Store in cool and dry area
Certification	ISO.