BCIP Na) 5-Bromo-4-chloro-3-indolyl phosphate disodium salt CAS:102185-33-1

Effect:

BCIP is a colorless compound that is converted into a blue-purple precipitate upon the action of alkaline phosphatase. This color change allows for visual detection and quantification of the enzyme's activity.

Applications:

Enzyme assays: BCIP is used in various biochemical assays to measure the activity of alkaline phosphatase. This includes detecting phosphatase activity in cell lysates, purified enzymes, or in vitro assays.

Immunohistochemistry: BCIP can be used to visualize alkaline phosphatase-labeled antibodies in tissue sections or on cell surfaces. This technique is often used in immunostaining protocols.

Blotting techniques: BCIP is used in Western blotting, Southern blotting, and Northern blotting to detect specific proteins or nucleic acids that have been labeled with alkaline phosphatase-conjugated probes.

In situ hybridization: BCIP can be used to visualize gene expression patterns in tissues or cells through in situ hybridization methods.

Diagnostic assays: BCIP is utilized as a substrate in various diagnostic kits for the detection of alkaline phosphatase. These kits are used in clinical laboratories for disease diagnosis and monitoring.