BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine salt CAS:6578-06-9

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt is a chemical compound commonly used in molecular biology and biochemistry research. It is often used in enzyme-linked immunosorbent assays (ELISA) and protein labeling techniques.

This compound is a substrate for alkaline phosphatase, an enzyme widely used in molecular biology for detecting the presence of specific molecules or proteins through colorimetric or chemiluminescent reactions.

When the alkaline phosphatase enzyme is present, it cleaves the phosphate groups from 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, resulting in the formation of a blue or purple dye. This color change can be easily detected and quantified, enabling the measurement of enzyme activity or the presence of target molecules.

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Application and Effect

Western blotting: BCIP is used to detect and visualize proteins transferred onto membranes after gel electrophoresis. Alkaline phosphatase-conjugated secondary antibodies are used to bind to the primary antibodies, and subsequent incubation with BCIP results in the development of a blue precipitate at the site of protein bands.

Immunohistochemistry: BCIP is utilized in immunohistochemical staining techniques to detect the presence of specific proteins in tissues. Alkaline phosphatase-conjugated secondary antibodies are used to bind to primary antibodies, and BCIP treatment generates a blue color in the presence of the enzyme, indicating protein localization within the tissue sample.

In situ hybridization: BCIP is also used for detecting nucleic acid sequences in fixed tissues or cells. Hybridization probes are labeled with alkaline phosphatase, and upon addition of BCIP, the presence of the target mRNA or DNA is indicated by the development of a blue color.

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Additional Information:

Composition	C8H6BrClNO4P.C7H9N
Assay	99%
Appearance	White powder
CAS No.	6578-06-9
Packing	Small and bulk
Shelf Life	2 years
Storage	Store in cool and dry area
Certification	ISO.