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BES CAS:10191-18-1 Manufacturer Price

N,N-Bis(hydroxyethyl)-2-aminoethanesulfonic acid, also known as BES or N,N-Bis(2-hydroxyethyl)aminoethanesulfonic acid, is a chemical compound that is commonly used as a buffering agent in various scientific and industrial applications.

BES is a zwitterionic compound, meaning it has both positive and negative charges within its structure. This property allows it to effectively maintain a stable pH in solutions.

BES has a pKa value of approximately 7.4, making it particularly useful for buffering at physiological pH levels. It is often utilized in biological and biochemical experiments, such as protein purification, enzyme reactions, and cell culture, where maintaining a specific pH is critical.

Additionally, BES is widely used in electrophoresis techniques, as it helps maintain the pH necessary for the separation and analysis of charged biomolecules, such as proteins and nucleic acids.


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Application and Effect

pH Buffering: BES has effective buffering capacity at a pH range around 6.4 to 7.8. It helps maintain a stable pH by regulating the concentration of hydrogen ions in a solution. This makes it particularly useful in biological and chemical assay systems where maintaining a specific pH is essential.

Protein Stabilization: BES is commonly employed in protein purification and storage procedures. Its buffering properties can help maintain the pH within the optimum range for protein stability and prevent denaturation or degradation of proteins.

Enzyme Reactions: BES is often utilized as a buffering agent in enzymatic reactions. It helps maintain the optimal pH for enzyme activity, ensuring that the reaction proceeds efficiently.

Cell Culture: BES is utilized in cell culture applications, especially in mammalian cell lines. It helps maintain the pH of the growth medium, which is crucial for cell viability and optimal cellular functions.

Electrophoresis: BES is used as a buffering agent in electrophoresis techniques for the separation and analysis of biomolecules, including proteins and nucleic acids. It ensures that the separation occurs within the desired pH range, allowing for accurate analysis.

Product Packing:

6892-68-8-3

Additional Information:

Composition C6H15NO5S
Assay 99%
Appearance White powder
CAS No. 10191-18-1
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

 


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