BIOTINAMIDOCAPROYL HYDRAZIDE CAS:109276-34-8

Biotinamidocaproyl hydrazide has numerous applications across molecular biology, biochemistry, and diagnostic fields. One of its primary uses is in the development of bioconjugates for use in affinity purification techniques. By attaching biotinamidocaproyl hydrazide to specific biomolecules, scientists can utilize the high affinity between biotin and streptavidin or avidin to purify proteins or other macromolecules from complex mixtures. This method enhances the efficiency and specificity of protein isolation and characterization. In addition, this compound serves as a valuable tool in assay development, particularly in enzyme-linked immunosorbent assays (ELISA) or other immunoassays. By incorporating biotin into these assays, researchers can improve sensitivity and detection limits, enabling the identification of low-abundance targets. The stable interaction between biotin and its binding partners facilitates signal amplification, which is crucial for accurate quantification. Moreover, biotinamidocaproyl hydrazide can be utilized in the creation of biotinylated probes for imaging applications. By linking it to fluorescent dyes or other tracers, scientists can track cellular processes or visualize biomolecule interactions within living cells. This application is particularly valuable in studying cell signaling pathways and understanding disease mechanisms. Furthermore, the versatility of biotinamidocaproyl hydrazide allows for modifications that can enhance its properties, such as solubility or stability. This adaptability promotes its integration into various experimental designs, ranging from drug delivery systems to the development of biosensors. In summary, the multifunctional capabilities of biotinamidocaproyl hydrazide underscore its importance in advancing research methodologies in the life sciences, providing innovative solutions for protein analysis, diagnostic assays, and molecular imaging.