Bis[2-Hydroxyethyl] imino Tris-(Hydroxymethyl)-methane CAS:6976-37-0

Buffering Agent: Bicine acts as a buffering agent and helps maintain a stable pH in aqueous solutions. It has an effective buffering range of pH 7.6 to 9.0, making it useful in biochemical and biological applications.

Enzyme Assays: Bicine is commonly used in enzyme assays and biochemical experiments due to its compatibility with enzymatic reactions. It helps maintain the optimum pH for enzyme activity, allowing accurate measurement of enzyme kinetics and activity.

Cell Culture Media: Bicine is used in cell culture media as a pH regulator to maintain the optimal growth conditions for various cell types. It provides a stable environment for cell growth and ensures that the pH remains within the desired range.

Protein Purification: Bicine is used in protein purification processes, particularly during ion exchange chromatography. It aids in protein elution and provides buffering capacity to maintain the stability of the purified protein.

Electrophoresis: Bicine is commonly used as a buffering agent in gel electrophoresis techniques, such as polyacrylamide gel electrophoresis (PAGE). It helps to maintain a stable pH in the gel, which is crucial for separating and analyzing proteins and nucleic acids.

Pharmaceutical Formulations: Bicine is used in the formulation of pharmaceutical products to adjust and stabilize the pH of the solution. It can be found in various medications, including liquid formulations, injectables, and topical preparations.