CHES Na CAS:103-47-9 Manufacturer Price

2-(Cyclohexylamino)ethanesulfonic acid is a chemical compound with the molecular formula C10H21NO3S. It is also known by its abbreviation CHES. CHES is a sulfonic acid derivative that contains both an amino group and a sulfonic acid group in its structure.

CHES is commonly used as a buffering agent in biochemical and biological research. It is pH-stable and maintains a consistent pH environment, particularly in laboratory settings involving enzymatic reactions or protein studies. CHES has a pKa of 9.3, making it an effective buffer around pH 9.

Its unique chemical structure and properties make CHES useful in various applications, such as in the preparation of buffer solutions for electrophoresis, enzyme assays, and cell culture media. It is often preferred for applications that require a pH range of 8.5 to 10.

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Application and Effect

Buffering: CHES is commonly used as a buffering agent to maintain a stable pH in biological experiments and enzymatic reactions. It is especially useful in applications requiring a pH range of 8.5 to 10.

Electrophoresis: CHES is frequently used as a buffer in electrophoresis techniques, such as SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis), to separate proteins based on their molecular weight.

Enzyme assays: CHES is utilized in enzyme assays to maintain an optimal pH for enzyme activity. It helps to ensure that enzymatic reactions occur under controlled and reliable conditions.

Cell culture media: CHES is sometimes included in cell culture media for various cell types as a pH-regulating component. It helps maintain physiological pH levels, which is critical for proper cell growth and function.

Protein studies: CHES is often utilized in protein purification and protein crystallization experiments. Its buffering properties help maintain the stability and integrity of proteins during these processes.

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Additional Information:

Composition	C8H17NO3S
Assay	99%
Appearance	White powder
CAS No.	103-47-9
Packing	Small and bulk
Shelf Life	2 years
Storage	Store in cool and dry area
Certification	ISO.