Disodium 4-nitrophenylphosphate CAS:4264-83-9

The main effect and application of disodium 4-nitrophenylphosphate is its use as a chromogenic substrate for alkaline phosphatase enzyme assays. When this compound is incubated with alkaline phosphatase, the enzyme cleaves the phosphate group from disodium 4-nitrophenylphosphate, resulting in the formation of 4-nitrophenol.

The produced 4-nitrophenol is a yellow-colored compound that can be detected spectrophotometrically at 405 nm. This color change is used to quantitatively measure the activity of alkaline phosphatase in samples, such as biological fluids or cell lysates.

The application of disodium 4-nitrophenylphosphate in enzyme assays provides a convenient method for measuring alkaline phosphatase activity. This enzyme is involved in various biological processes and is commonly used as a marker for tissue differentiation, liver function, and bone metabolism.

Additionally, disodium 4-nitrophenylphosphate can be used as a substrate for other phosphatases, such as acid phosphatase or protein phosphatases, depending on the experimental setup and research goal.