DNase I, from Bovine Pancreas CAS:9003-98-9

DNase I, derived from bovine pancreas, is an enzyme that specifically hydrolyzes DNA molecules. It cleaves the phosphodiester bonds present in the DNA backbone, resulting in the degradation of the DNA into smaller fragments. DNase I is widely used in molecular biology research, particularly in applications involving DNA isolation, purification, and manipulation. It is employed to remove genomic DNA contamination from RNA samples, to degrade DNA in protein-DNA complexes, and to fragment DNA for various experiments such as DNA footprinting and chromatin analysis. DNase I also finds applications in medical diagnostics, where it is used to detect and quantify cell-free DNA in circulating blood, as well as in forensic science for the analysis of DNA evidence.

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Application and Effect

DNase I, derived from bovine pancreas, is an enzyme that specifically cleaves DNA molecules by hydrolyzing the phosphodiester bonds in the DNA backbone. The main effect of DNase I is the degradation and fragmentation of DNA.

The primary application of DNase I is in molecular biology research. It is commonly used in various experiments to isolate and purify DNA, remove genomic DNA contamination from RNA samples, and manipulate DNA. DNase I is particularly effective in degrading DNA in protein-DNA complexes, allowing researchers to study protein-DNA interactions or remove DNA-bound proteins.

In addition, DNase I is frequently utilized in DNA footprinting assays, which help identify regions of DNA that are bound by specific proteins. By treating a DNA sample with DNase I and subsequently analyzing the resulting DNA fragments, researchers can determine the binding sites of DNA-binding proteins.

DNase I is also used in chromatin analysis, where it aids in the digestion of chromatin (DNA and associated proteins) to study its structure and organization. By digesting chromatin with DNase I, researchers can identify regions that are more accessible and sensitive to cleavage, providing insights into gene regulation and chromatin structure.

Furthermore, DNase I has applications in medical diagnostics, specifically in detecting and quantifying cell-free DNA in circulating blood. By using DNase I to digest non-circulating DNA, it is possible to analyze and detect specific DNA sequences associated with diseases or genetic abnormalities.