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4-Nitrophenyl-alpha-D-glucopyranoside CAS:3767-28-0
4-Nitrophenyl-alpha-D-glucopyranoside is a chemical compound that is commonly used in biochemical experiments and assays. It is a substrate that can be cleaved by certain enzymes, such as glycosidases, to release a detectable product. Its structure consists of a glucose molecule (alpha-D-glucose) linked to a 4-nitrophenyl group. This compound is often used to study and measure the activity of enzymes involved in carbohydrate metabolism and glycosylation processes.
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Tricine CAS:5704-04-1 Manufacturer Price
Tricine is a zwitterionic organic compound with the chemical formula C6H13NO5S. It is widely used as a buffering agent, primarily in biochemical and biological applications. Tricine’s distinguishing feature is its unique buffering capacity at a slightly acidic pH range, making it particularly useful in experiments that require a stable and precise pH environment. It is commonly used in protein electrophoresis, molecular biology techniques, enzymatic assays, and cell culture media. Tricine helps maintain optimal conditions for various biological processes, ensuring accurate and reliable results in research and analysis.
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Egtazic acid CAS:67-42-5 Manufacturer Price
Ethylenebis(oxyethylenenitrilo)tetraacetic acid (EGTA) is a chelating agent commonly used in biological and chemical research. It is a synthetic compound that is derived from ethylenediamine and ethylene glycol. EGTA has a high affinity for divalent metal ions, particularly calcium, and is widely used to chelate and sequester these ions in various applications such as in cell culture, enzyme assays, and molecular biology techniques. By binding to calcium and other metal ions, EGTA helps to regulate their concentrations, thus influencing various biochemical processes.
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X-GAL CAS:7240-90-6 Manufacturer Price
5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) is a common chromogenic substrate used in molecular biology and biochemistry applications. It is widely used for the detection of the lacZ gene, which encodes the enzyme β-galactosidase.
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1,2,3,4,6-Penta-O-acetyl-D-mannopyranose CAS:25941-03-1
1,2,3,4,6-Penta-O-acetyl-D-mannopyranose is a chemical compound derived from D-mannose, a simple sugar. It is a derivative where acetyl groups are attached to five of the six hydroxyl groups present in the mannose molecule. This acetylated form of D-mannose is commonly used in organic synthesis and chemical research as a building block or starting material for the synthesis of more complex molecules. The acetyl groups provide stability and can alter the reactivity and properties of the compound.
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1,2,3,4-Di-O-Isopropylidene-alpha-D-galactopyranose CAS:4064-06-6
1,2:3,4-Di-O-isopropylidene-D-galactopyranose is a chemical compound that belongs to the family of galactopyranose derivatives. It is commonly used in organic chemistry as a protecting agent for the hydroxyl groups present in sugars, specifically galactose. The compound is synthesized by reacting D-galactose with acetone to form a diacetone derivative, which is then treated with acid to form the di-O-isopropylidene derivative. This derivative protects the hydroxyl groups, preventing unwanted reactions during chemical synthesis, and can be selectively removed to regenerate the original compound. Its compact structure and stability make it advantageous in various applications within the field of organic synthesis.
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PNPG CAS:3150-24-1 Manufacturer Price
PNPG, or p-nitrophenyl β-D-glucopyranoside, is a small molecule substrate often used in biochemical assays to measure the activity of glucosidase enzymes. It is colorless and non-fluorescent, but upon hydrolysis by glucosidase, it is converted into p-nitrophenol, which has a yellow color and is easily detectable spectrophotometrically.
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ONPG CAS:369-07-3 Manufacturer Price
O-nitrophenyl-β-D-galactopyranoside (ONPG) is a synthetic substrate used in biochemical and molecular biology assays to measure the activity of the enzyme β-galactosidase. It is commonly used in assays to detect gene expression in bacterial systems, such as Escherichia coli.ONPG is a colorless compound that is cleaved by β-galactosidase, resulting in the release of a yellow compound, o-nitrophenol. The yellow color produced can be measured spectrophotometrically, providing an indirect measurement of enzyme activity.The assay using ONPG is often referred to as the ONPG assay and is a widely used method in molecular biology research to assess the expression levels of genes controlled by the lac operon in bacterial cells.
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Nitrotetrazolium Blue Chloride CAS:298-83-9
Nitrotetrazolium Blue Chloride (NBT) is a redox indicator commonly used in biological and biochemical assays. It is a pale yellow powder that turns blue when reduced, making it useful for detecting the presence of certain enzymes and metabolic activity.
NBT reacts with electron carriers and enzymes such as dehydrogenases, which are involved in various cellular processes. When NBT is reduced by these enzymes, it forms a blue formazan precipitate, allowing for visual or spectrophotometric detection.
This reagent is commonly used in assays such as the NBT reduction test, where it is used to assess the respiratory burst activity of immune cells. It can also be used to study enzyme activities and metabolic pathways in research pertaining to oxidative stress, cell viability, and cell differentiation.
NBT has found applications in various fields, including microbiology, immunology, and cell biology. It is versatile, relatively stable, and easy to use, making it a popular choice for many experimental protocols.
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HATU CAS:148893-10-1 Manufacturer Price
HATU (1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate) is a commonly used coupling reagent in peptide synthesis and organic chemistry.
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D-fucose CAS:3615-37-0 Manufacturer Price
D-fucose is a monosaccharide, specifically a six-carbon sugar, that belongs to the group of simple sugars called hexoses. It is an isomer of glucose, differing in the configuration of one hydroxyl group.
D-fucose is naturally found in various organisms, including bacteria, fungi, plants, and animals. It plays important roles in several biological processes, such as cell signaling, cell adhesion, and glycoprotein synthesis. It is a component of glycolipids, glycoproteins, and proteoglycans, which are involved in cell-to-cell communication and recognition.
In humans, D-fucose is also involved in the biosynthesis of important glycan structures, such as Lewis antigens and blood group antigens, which have implications in blood transfusion compatibility and disease susceptibility.
D-fucose can be obtained from various sources, including seaweed, plants, and microbial fermentation. It is utilized in research and biomedical applications, as well as in the production of certain pharmaceuticals and therapeutic compounds.
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DDT CAS:3483-12-3 Manufacturer Price
DL-Dithiothreitol, also known as DTT, is a reducing agent commonly used in biochemical and molecular biology research. It is a small molecule with a thiol (sulfur-containing) group on each end.
DTT is frequently used to break disulfide bonds in proteins, which helps to unfold or denature them. This reduction of disulfide bonds is important in various laboratory procedures such as protein purification, gel electrophoresis, and protein structure studies. DTT can also be used to protect thiol groups and prevent oxidation during experimental procedures.
DTT is typically added to experimental solutions in small concentrations, and its activity is dependent on the presence of oxygen. It is important to handle DTT carefully as it is sensitive to air, heat, and moisture, which can reduce its effectiveness.
