Fluorescein Isothiocyante CAS:3326-32-7

Fluorescence properties: FITC has a high quantum yield and emits bright green fluorescence upon excitation at around 488 nm. Its fluorescence properties make it suitable for detection using various imaging techniques, such as fluorescence microscopy, flow cytometry, and fluorescence spectroscopy.

Covalent binding: FITC contains an isothiocyanate group that allows it to covalently bind to amino groups present in proteins, antibodies, and other biomolecules. This covalent binding ensures stable and specific labeling of target molecules, enabling their detection and visualization.

Immunofluorescence: FITC-labeled antibodies are commonly used in immunofluorescence assays to detect specific antigens or proteins in cells and tissues. The bright green fluorescence emitted by FITC enables researchers to localize and visualize the distribution of target molecules within biological samples.

Flow cytometry: FITC-labeled antibodies or other fluorochrome-conjugated biomolecules are widely used in flow cytometry to analyze and sort cells based on their surface markers or intracellular components. FITC is compatible with the commonly used 488 nm laser utilized in flow cytometry instruments.

Protein interaction studies: FITC can be used in protein-protein interaction studies to assess the binding and interaction of proteins. By labeling specific proteins with FITC, researchers can analyze their interactions using techniques like fluorescence resonance energy transfer (FRET) or fluorescence polarization.

Biomolecule tracking: FITC can be used to trace and track the movement of biomolecules within living cells or tissues. By labeling molecules of interest with FITC, researchers can study their localization, dynamics, and trafficking patterns in real-time using fluorescence microscopy.

Diagnostic applications: FITC-labeled probes or antibodies are used in diagnostics for various applications, including detection of specific antigens, infectious agents, or biomarkers in patient samples. These fluorescent probes provide sensitive and specific detection, allowing for rapid and accurate diagnosis.