Gly-Pro p-nitroanilide p-toluenesulfonate salt CAS:65096-46-0

Effect: Gly-Pro p-nitroanilide p-toluenesulfonate salt is specifically designed to be a substrate for proline-specific peptidases. These enzymes cleave the peptide bond between glycine (Gly) and proline (Pro) residues, releasing p-nitroanilide (pNA) as a product. The pNA molecule absorbs light at a specific wavelength, allowing for the measurement of the released pNA and estimation of the enzyme activity.

Application: Gly-Pro p-nitroanilide p-toluenesulfonate salt is used in various research areas, including biochemistry, enzymology, and drug discovery, for the following applications:

Enzyme activity assays: It is widely used to measure the activity of proline-specific peptidases, including dipeptidyl peptidases (DPPs) and prolyl endopeptidases (PEPs).

Screening enzyme inhibitors: Gly-Pro p-nitroanilide p-toluenesulfonate salt can be used to evaluate the inhibitory potential of compounds or drugs on proline-specific peptidase activity. Any decrease in the release of pNA indicates the presence of an inhibitor.

Enzyme kinetics studies: By varying the concentration of Gly-Pro p-nitroanilide p-toluenesulfonate salt and measuring the rate of pNA release, enzyme kinetics parameters such as Km (Michaelis-Menten constant) and Vmax (maximum velocity) can be determined.

Diagnostic applications: The activity of proline-specific peptidases can be relevant in diagnostic tests for various diseases and conditions, such as infections and cancers. Gly-Pro p-nitroanilide p-toluenesulfonate salt can be used as a tool to assess the activity levels of these enzymes in patient samples.