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Guanidine Thiocyanate Sulfate CAS:593-84-0

Guanidine thiocyanate sulfate is a chemical compound derived from guanidine, thiocyanate, and sulfate ions. It is commonly used in molecular biology and biochemistry research as a chaotropic agent.

Guanidine thiocyanate sulfate is often utilized in DNA and RNA isolation protocols to disrupt cellular membranes and denature proteins, allowing for efficient extraction of nucleic acids. It helps in the solubilization of DNA and RNA, enabling their isolation and purification from various biological samples.

Due to its chaotropic properties, guanidine thiocyanate sulfate assists in breaking hydrogen bonding and other non-covalent interactions, facilitating the separation of nucleic acids from proteins, lipids, and other cellular components. It also helps in inhibiting RNase activity, ensuring the integrity and purity of the extracted nucleic acids.

 


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Application and Effect

Effects:

Denaturation: Guanidine thiocyanate sulfate disrupts the native structure of proteins, DNA, and RNA, leading to their denaturation.

Solubilization: The compound's strong chaotropic properties enable it to dissolve biomolecules, making it easier to isolate and purify them.

Applications:

Nucleic acid isolation: Guanidine thiocyanate sulfate is commonly used in various nucleic acid extraction protocols, such as DNA or RNA purification, to help break down cellular components and separate the genetic material from the rest of the cellular matrix.

Protein purification: It can also be used in protein purification methods, such as the isolation of recombinant proteins or protein complexes, as it effectively disrupts the structure of proteins and solubilizes them.

Enzyme inactivation: Due to its denaturing properties, guanidine thiocyanate sulfate can be used to inactivate enzymes, which is useful in some laboratory techniques or storage of samples.

Product Packing:

试剂包装2

Additional Information:

Composition CH5N3.CHNS
Assay 99%
Appearance White powder
CAS No. 593-84-0
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

 


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