HEPES-Na CAS:75277-39-3 Manufacturer Price

Buffering agent: HEPES sodium salt is commonly used as a buffering agent in biological and biochemical experiments. It helps maintain a stable pH range, particularly in the physiological range (pH 7.2-7.6). Its buffering capacity makes it valuable in maintaining proper conditions for various enzymatic reactions, cell culture, and molecular biology techniques.

Cell culture: HEPES sodium salt is extensively used as a buffering agent in cell culture media. The ability to maintain a stable pH is essential for the proper growth and viability of cells. HEPES is often preferred over other buffering agents since it does not show significant changes in pH when exposed to atmospheric CO2.

Enzyme studies: HEPES sodium salt is particularly useful in enzymatic studies where a constant and controlled pH environment is required. It prevents drastic fluctuations in the pH during enzymatic reactions, ensuring optimal enzyme activity.

Electrophoresis: HEPES sodium salt is commonly used as a buffering agent in various electrophoretic techniques such as polyacrylamide gel electrophoresis (PAGE) and agarose gel electrophoresis. It helps maintain the pH of the buffer, which is crucial for separating and analyzing nucleic acids and proteins.

Biochemical assays: HEPES sodium salt is often utilized in a variety of biochemical assays, including enzyme assays, protein quantification assays, and spectrophotometric assays. It helps maintain the desired pH range required for accurate and reproducible results.

Drug formulation: HEPES sodium salt is also used in pharmaceutical formulations as a buffering agent to stabilize drug formulations and maintain the desired pH range.