Hygromycin B CAS:31282-04-9

When using hygromycin B in cell culture or molecular biology experiments, it is essential to handle the compound with care and follow proper safety protocols. To prepare a hygromycin B solution for cell selection, dissolve the powder in an appropriate solvent such as water or ethanol to achieve the desired concentration recommended for your specific application. Add the hygromycin B solution to the cell culture media at the appropriate concentration to selectively kill non-resistant cells, allowing only the transformed or resistant cells to survive and proliferate. Monitor the cell culture regularly to ensure proper selection and growth of the desired cells. For molecular biology applications, include hygromycin B in the growth media of transformed cells to maintain selection pressure and stable expression of resistance genes. Perform regular checks and assays to confirm the stability and efficiency of hygromycin B selection in maintaining the desired genetic modifications. Always refer to the product information and technical guidelines provided by the manufacturer for specific instructions on handling, preparation, and usage of hygromycin B in your experiments. Remember to wear appropriate personal protective equipment and work in a designated laboratory area when working with this antibiotic to ensure safety and prevent contamination.