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Iodonitrotetrazolium chloride CAS:146-68-9 Manufacturer Price

Iodonitrotetrazolium chloride, often abbreviated as INT, is a pale yellow dye commonly used in biological and biochemical research. It is a redox indicator that changes color when reduced, making it useful for detecting the presence of certain enzymes or for visualizing cellular metabolic activity. INT is often used in assays to measure dehydrogenase activity and cell viability. When reduced, it forms a formazan product that is generally red in color, allowing for easy detection and quantification in various types of assays.


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Application and Effect

Iodonitrotetrazolium chloride (INT) is frequently used to detect the activity of dehydrogenase enzymes in biological systems. These enzymes are involved in cellular metabolism and play a crucial role in various biochemical pathways. INT is especially useful in assays designed to assess cell viability and metabolic activity. When INT is added to a test sample, the reduction of the dye by dehydrogenase enzymes results in the formation of a red formazan product. The intensity of the red color can be measured spectrophotometrically, providing a quantitative measure of enzyme activity and cell viability. This makes INT valuable in a wide range of applications, including cell proliferation assays, microbial viability assays, and evaluation of drug effects on cellular metabolism. Its use in research and diagnostic assays has contributed to a better understanding of physiological and pathological processes at the cellular level.

Product Packing:

试剂包装2

Additional Information:

Composition C19H13ClIN5O2
Assay 99%
Appearance White powder
CAS No. 146-68-9
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

 


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