MES HEMISODIUM SALT CAS:117961-21-4

Buffering agent: MES hemisodium salt is used to maintain a stable pH in experimental solutions. It has a pKa value of 6.1, making it effective in the pH range of 5.05 to 6.77. This makes it suitable for a variety of applications, including cell culture, enzyme assays, and protein purification.

Stabilization of enzymes and proteins: MES hemisodium salt is often used to stabilize the activity and structure of enzymes and proteins during experiments. It helps in maintaining the desired pH conditions, thus preventing denaturation and degradation.
Electrophoresis: MES hemisodium salt is commonly used as a buffer in agarose and polyacrylamide gel electrophoresis. It helps in maintaining the optimal pH for the separation of DNA, RNA, and proteins.
Cell culture: MES hemisodium salt is used in cell culture media to maintain a suitable pH for cell growth and viability. It is particularly useful for cell lines that require a slightly acidic pH environment.
Molecular biology techniques: MES hemisodium salt is used in various molecular biology techniques, such as DNA and RNA extraction, PCR, and DNA sequencing. It ensures the stability and integrity of nucleic acids during these procedures.

Plant growth media: MES hemisodium salt is also used in plant tissue culture media to establish optimal pH conditions for the growth and development of plant cells and tissues.