MES monohydrate CAS:145224-94-8

Buffering agent: MES monohydrate is primarily used as a buffering agent to maintain a stable pH in experimental setups. Its effective buffering range is around pH 5.5 to 6.7. It resists changes in pH caused by the addition of acids or bases, making it useful in various biochemical and biological studies.

Enzyme studies: MES monohydrate is commonly used in studying the activity and kinetics of enzymes. Its buffering capacity at the pH range compatible with many enzyme systems makes it an ideal choice for these studies.

Protein purification: During protein purification processes, maintaining a stable pH is critical for protein stability and activity. MES monohydrate can be used as a buffering agent during various steps of protein purification, including protein extraction, purification, and storage.

Gel electrophoresis: MES monohydrate is often used as a buffer in gel electrophoresis to maintain a stable pH during the separation and analysis of proteins or nucleic acids. It provides the necessary pH conditions for optimum separation and migration of molecules through the gel matrix.

Cell culture: Maintaining a stable pH is essential for cell culture experiments. MES monohydrate can be used as a buffering agent in cell culture media to ensure optimal growth conditions for cells.

Chemical reactions: MES monohydrate has been used in various chemical reactions that require a specific pH range. Its buffering capacity helps to maintain the desired pH for the chemical reaction to proceed efficiently.