MOPS CAS:1132-61-2

3-(N-Morpholino)propanesulfonic acid, commonly referred to as MOPS, is an organosulfonic acid that serves as an important buffering agent in numerous biological and biochemical applications. The compound features a morpholine ring, which contributes to its buffering capacity, and a sulfonic acid group, providing strong acidity. With the molecular formula C7H15NO4S, MOPS has a pKa value of approximately 7.15, making it particularly suitable for maintaining stable pH levels in experiments conducted at or near physiological pH. MOPS is widely utilized in molecular biology techniques, such as gel electrophoresis, where it helps maintain a consistent pH during the separation of nucleic acids and proteins. Its good solubility in aqueous solutions allows researchers to use it effectively in buffers for running gels, ensuring optimal conditions for the activity of enzymes and other biomolecules. Additionally, MOPS is often employed in cell culture media, as it helps stabilize pH during cellular metabolism, thus promoting cell growth and viability. One of the key advantages of using MOPS as a buffer is its low absorbance in the ultraviolet range, which reduces interference in spectrophotometric analyses. This characteristic makes it particularly useful in assays that involve UV detection. Furthermore, MOPS does not significantly impact enzymatic reactions, allowing for accurate measurements and reproducibility in experiments. In summary, MOPS is an essential tool in biological and biochemical research due to its ability to maintain stable pH levels, compatibility with macromolecules, and low UV absorbance. Its versatility spans various applications, from protein purification to molecular cloning, solidifying its status as a standard buffer in laboratories worldwide. As research continues to advance, MOPS remains a reliable choice for scientists aiming to achieve precise control over experimental conditions.