MOPS sodium salt CAS:71119-22-7

Effect:

Buffering Capacity: MOPS sodium salt effectively maintains the desired pH range by accepting or donating protons, thereby resisting changes in pH caused by added acids or bases. It is particularly effective in the pH range of approximately 6.5 to 7.9, making it suitable for a wide range of biological applications.

Applications:

Protein Research: MOPS sodium salt is commonly used as a buffering agent in protein research experiments, such as protein purification, protein characterization, and protein crystallization. It helps maintain optimal conditions for protein stability, enzymatic activity, and protein folding studies.

Cell Culture: MOPS sodium salt is used in cell culture media to maintain a stable pH environment, which is critical for the growth and viability of cells. It is often preferred over other buffering agents due to its minimal cytotoxic effects on cells.

Gel Electrophoresis: MOPS sodium salt is used as a buffering agent in polyacrylamide gel electrophoresis (PAGE) systems. It helps maintain a constant pH during the separation of proteins or nucleic acids, allowing for accurate migration and resolution.

Enzymatic Reactions: MOPS sodium salt is frequently used in enzymatic reactions as a buffering agent to optimize the pH conditions required for enzymatic activity. It ensures that the enzymatic reaction proceeds efficiently and accurately.

Nucleic Acid Research: MOPS sodium salt is used in nucleic acid research applications, such as DNA and RNA isolation, purification, and analysis. It helps maintain a stable pH during enzymatic reactions and gel electrophoresis, which are essential steps in nucleic acid studies.