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MOPS sodium salt CAS:71119-22-7

MOPS sodium salt, also known as3-(N-morpholino)propanesulfonic acid sodium salt, is a commonly used buffering agent in biochemical and molecular biology research. It is used to maintain a stable pH range and create ideal conditions for enzymatic reactions, protein stability, and cell culture growth. MOPS sodium salt is particularly effective in providing buffering capacity in the pH range of approximately 6.5 to 7.9. It is widely utilized in protein purification processes, gel electrophoresis, enzyme studies, and cell culture experiments.


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Application and Effect

Effect:

Buffering Capacity: MOPS sodium salt effectively maintains the desired pH range by accepting or donating protons, thereby resisting changes in pH caused by added acids or bases. It is particularly effective in the pH range of approximately 6.5 to 7.9, making it suitable for a wide range of biological applications.

Applications:

Protein Research: MOPS sodium salt is commonly used as a buffering agent in protein research experiments, such as protein purification, protein characterization, and protein crystallization. It helps maintain optimal conditions for protein stability, enzymatic activity, and protein folding studies.

Cell Culture: MOPS sodium salt is used in cell culture media to maintain a stable pH environment, which is critical for the growth and viability of cells. It is often preferred over other buffering agents due to its minimal cytotoxic effects on cells.

Gel Electrophoresis: MOPS sodium salt is used as a buffering agent in polyacrylamide gel electrophoresis (PAGE) systems. It helps maintain a constant pH during the separation of proteins or nucleic acids, allowing for accurate migration and resolution.

Enzymatic Reactions: MOPS sodium salt is frequently used in enzymatic reactions as a buffering agent to optimize the pH conditions required for enzymatic activity. It ensures that the enzymatic reaction proceeds efficiently and accurately.

Nucleic Acid Research: MOPS sodium salt is used in nucleic acid research applications, such as DNA and RNA isolation, purification, and analysis. It helps maintain a stable pH during enzymatic reactions and gel electrophoresis, which are essential steps in nucleic acid studies.

Product Packing:

6892-68-8-3

Additional Information:

Composition C7H16NNaO4S
Assay 99%
Appearance White powder
CAS No. 71119-22-7
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

 


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