N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine sodium salt CAS:102062-46-4

N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine sodium salt is a chemical compound commonly used in various laboratory applications. It is a water-soluble derivative of tetramethylbenzidine (TMB) that contains a sulfopropyl group.

This compound is often employed as a substrate in colorimetric assays to detect the presence of enzymes such as peroxidases or horseradish peroxidase (HRP). When these enzymes are present, they can catalyze a reaction with N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine sodium salt, leading to the generation of a colored product. The intensity of the color is directly proportional to the enzymatic activity, allowing for the quantification of the enzyme or the detection of specific substrates.

The water solubility of N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine sodium salt makes it convenient for use in aqueous solutions and compatible with various assay systems. Its sulfopropyl group enhances the solubility and stability of the compound, enabling efficient and reliable detection in enzymatic assays.

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Application and Effect

Immunohistochemistry (IHC): STMB is used as a chromogenic substrate in IHC to visualize the presence of specific antigens in tissue samples. The resulting color allows for the identification and localization of the targeted proteins.

ELISA: STMB is widely used in ELISA and enzyme immunoassays (EIA) to detect and quantify target analytes such as proteins, hormones, and antibodies. The colorimetric reaction facilitated by STMB provides a measurable signal for the presence and concentration of the analyte.

Western blotting: STMB is used as a substrate for HRP-conjugated secondary antibodies in western blotting to visualize the presence of target proteins. The resulting color allows for the specific detection of proteins of interest on the membrane.

Enzyme activity assays: STMB can be utilized to measure the peroxidase activity of different enzymes. By monitoring the generation of color, researchers can determine the enzymatic activity and evaluate the efficiency of enzymatic reactions.

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Additional Information:

Composition	C19H25N2NaO3S
Assay	99%
Appearance	White powder
CAS No.	102062-46-4
Packing	Small and bulk
Shelf Life	2 years
Storage	Store in cool and dry area
Certification	ISO.